from: innovations report (Pressemitteilung), Germany - Sep 8, 2008

 

Root-knot Nematode Genome Sequenced


Iowa State University researchers have contributed to the release of the annotated genome of one of the most destructive nematodes: Meloidogyne incognita -- the southern root-knot nematode, as reported recently in the journal Nature Biotechnology.

Sequencing the genome is a critical step toward comprehensively understanding how the organism works and may pave the way for research on ways to fight the pest.

"This is considered to be one of, if not the most important plant-parasitic nematode species across the world," said Thomas Baum, professor and chair of plant pathology and head of Iowa State University's contribution to the genome sequence project.

Root-knot nematodes are so important because they can be found almost anywhere in the world on almost any plant, he said. Nematodes are the most abundant animals on earth.

"Many of the nematodes that are really bad pathogens are very specialized on which plant they attack," said Baum. "This nematode has a huge host range. For us nematologists, it is very interesting and challenging to study."

Because the pest is so widespread, many nematologists around the world were eager to help with the project. The lead investigator was Pierre Abad of the Insitut National de Recherche Agronomiquea, a French research group, with help from researchers in Belgium, Holland, Great Britain, Switzerland, and Iowa State University and North Carolina State University in the US.

"Because it is such a worldwide problem, people are eager to contribute," Baum said. "Also, because it is the first plant-parasitic nematode to have its sequence released, people are very excited about it."

Chemical treatments for killing nematodes, called nematicides, are dangerous to humans and other animals so they've been restricted in use for decades. Technology for controlling nematodes has advanced little in the past three decades.

Besides being a devastating crop pathogen, Meloidogyne incognita has some remarkable biological adaptations that make it a fascinating organism to study.

Baum said that the sex of the tiny worms, or better the lack thereof is very intriguing. Only females reproduce and they do so without having sex, so it remains a puzzle why males of the species even exist. And since the females don't mate to reproduce, the offspring should be genetically identical to the mother -- like a clone - but they aren't. And as the offspring matures into males or females, some start as females and then change into males.

Baum's group included postdoctoral researcher Tarek Hewezi and assistant scientist Tom Maier from Iowa State. The three worked on a specific part of the genome and performed manual annotations of genes. Professor Davis and postdoctoral research associate, Noureddine Hamamouch, used the current known parasitism genes to identify the full suite or predicted parasitism genes in the root-knot nematode genome.

With this sequencing done, Baum thinks researchers can now try to understand this nematode. He also cautions that finding ways to control this pest will be a long process.

"For any effort in which you want to control the nematode, this is a great resource," Baum said. "But having the genome is only one of many steps in the right direction. Albeit, a very big one!"

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