NEMATOLOGY 100 MID-TERM EXAM -- 1
1/2 HOURS OCTOBER 31, 1990
1. (20 points) You are provided with nematodes extracted from a lawn at
Orchard Park Student Housing. Place an adult female plant-parasitic
nematode of any species in the Order Tylenchida on a microscope slide.
Heat-kill the nematode with gentle heat before applying a coverslip.
Place the slide on the compound microscope. Call the instructor for
examination of your nematode. If there is more than one nematode
present in the microscope field, indicate the specimen you wish to have
examined. Do not ask or expect the instructor to make the selection.
Only the nematode that you indicate initially will be considered. You
should not have to leave your bench position.
Grading:
adult female plant-parasite (Tylenchida), good condition - 20 pts
plant-parasite not in Tylenchida 8 pts
non-parasite, good condition - 4 pts
points off for damage, distortion, dry slides, etc.
2. (10 points) Describe the various sensory structures of tylenchid
nematodes and discuss their known or hypothesized function. Indicate by
a diagram the position of each structure in or on the nematode body.
3. (20 points) Provide the following information for each of the nematodes
listed. Answer by using the appropriate letter.
(i) Order: a) Tylenchida,
b) Rhabditida,
c) Diplogastrida,
d) Dorylaimida
(ii) Esophagus type : a) tylenchid - butting,
b) tylenchid - overlapping,
c) dorylaimid.
(iii) Number of ovaries (female): a) one,
b) two,
c) more than two,
d) none.
(iv) Known vector of a plant virus? a) no,
b) yes.
(v) Adult female feeding habit: a) migratory ectoparasite,
b) migratory endoparasite,
c) sedentary endoparasite.
(vi) Provide a recognized common name.
Belonolaimus longicaudatus
(i)
(ii)
(iii)
(iv)
(v)
(vi)
Paratrichodorus minor
(i)
(ii)
(iii)
(iv)
(v)
(vi)
Paratylenchus projectus
(i)
(ii)
(iii)
(iv)
(v)
(vi)
4. (15 points) Provide a generalized drawing of an adult female nematode
that is diovarial, amphidelphic, with a dorylaimid esophagus.
Label the features of the esophageal and stoma regions and of the
reproductive and digestive tracts.
5. (10 points) Provide details of the method in describing an appropriate
procedure for extracting:
a) Radopholus similis from banana roots
b) Second, 3rd, 4th and adult stages of Tylenchorhynchus dubius from
soil.
6. (15 points) Briefly discuss the economic importance, distribution, host
range, injury to host, relation to host tissue, biology as influenced by
physical environment, and control of the following:
a) Mesocriconema xenoplax
b) Hemicycliophora arenaria
c) Longidorus africanus
7. (10 points) Describe the geographic location and soil conditions that
favor the awl nematode, Dolichodorus heterocephalus. What crops are
damaged?
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