1. (20 points) You are provided with nematodes extracted from soil used
in an experiment to determine the effect of certain species on potato.
Place an adult female plant-parasitic nematode of any species in the
Order Tylenchida on a microscope slide.
Heat-kill the nematode with gentle heat before applying a coverslip.
Place the slide on the compound microscope.
Call the instructor for examination of your nematode. If there is more
than one nematode present in the microscope field, indicate the specimen
you wish to have examined. Do not ask or expect the instructor to make
the selection. Only the nematode that you indicate initially will be
considered. You should not have to leave your bench position.
Grading:
adult female plant-parasite (Tylenchida), good condition - 20 pts
plant-parasite not in Tylenchida 8 pts
non-parasite, good condition - 4 pts
points off for damage, distortion, dry slides, etc.
2. (15 points) Describe the structure and location of various chemosensory
structures of nematodes in the Tylenchida. What is their apparent
function. Discuss hypotheses of integration of signals from these
sensilla in orientation of the nematodes.
3. (15 points) Provide a generalized drawing of an adult female nematode
that is diovarial, amphidelphic, with evidence that functional males are
present in the population and that fertilization has occurred.
Indicate a postcorpus does not overlap the intestine.
Label the features of the esophageal and stoma regions and of the
reproductive and digestive tracts.
4. (13 points) Describe the parasitism of the lance nematode, Hoplolaimus columbus
on cotton and soybeans. What tissues are affected? What is the
overall impact on plant growth?
5. (12 points) The sting nematode, Belonolaimus longicaudatus, has been
detected this year in golf courses in Southern California. Provide the
following general and diagnostic characters of this nematode by circling
the appropriate choice:
(i) Order: a) Tylenchida
6. (15 points) What are the soil and climatic conditions in which the
burrowing nematode,
Radopholus similis is especially damaging to
citrus in Florida? Describe the nature and effect of the damage.
7. (10 points) List 5 diagnostic characteristics of organisms in the
Phylum Nematoda.
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