NEMATOLOGY 100
Laboratory Exercise 5
Rev. 01/01/2020
Purpose: Ectoparasitic nematodes: morphology, anatomy, feeding habits -- Tylenchidae, Dolichodoridae, Hoplolaimidae.
1.
Powerpoint presentation of characteristics.
2. Hand-pick live adult males and females of the true ectoparasites
from samples provided.
a) Tylenchus spp.
(small stomatostyle,
butting esophagus, monovarial,
pointed tail)
b) Tylenchorhynchus
sp. - stunt nematode - (short stomatostyle, butting esophagus, diovarial,
tapered to rounded tail)
Examine mounts of preserved specimens of
a) Dolichodorus heterocephalus - awl nematode - (rounded, offset lip region, long, slender stomatostyle, butting esophagus, diovarial, rounded tail)
b) Belonolaimus longicaudatus - sting nematode - long stomatostyle, overlapping esophagus, diovarial, pointed tail)
Draw the nematodes observing the following characteristic features:
a) stylet
length and morphology.
b) esophagus
type and anatomy, nature of postcorpus
interface with intestine.
c) male and
female reproductive structures.
d) general
shape when heat-relaxed.
3. Note the taxonomic position of these
nematodes in the Tylenchoidea.
4. Examine charts of other plant-parasitic
nematodes in these families.
5. Set up Baermann dishes to determine
nematode survival after exposure to plant residues (lab #4).
11. Nemabase Exercise
Review the host range of these nematodes. What are the indicated non-hosts or resistant plants?
From the Nemaplex main Menu, select Management and Decision Support, then Nemabase 2012.
Alternatively, you can access the data from the descriptive page for each nematode.
Note that a search on say "Tylenchus" will retrieve any record with"tylenchus: as part of the name, so you would need to sort the list and scroll down to that genus.