NEMATOLOGY 100
Laboratory Exercise 7
Rev. 01/01/2020
Purpose: Ectoparasitic nematodes: Orders Dorylaimida and Triplonchida
Morphology, anatomy,
feeding habits:
Longidoridae
(Dorylaimoidea, Dorylaimina,
Dorylaimida)
Trichodoridae
(Diphtherophoroidea, Diphtherophorina,
Triplonchida)
1. Powerpoint presentation and slides presentation of characteristics and virus transmission. (and of characteristics of nematodes in lab 6)
2. Hand-pick live adults of
Draw the nematodes, observing the following characteristic features:
a) spear length and morphology.
b) esophagus
type and anatomy, nature of postcorpus
interface with intestine.
c) male and
female reproductive structures.
d) general
shape when heat-relaxed.
3. Note the taxonomic position of these nematodes in the orders Dorylaimida and Triplonchida.
4. Examine descriptive charts of these plant-parasitic nematodes in the Dorylaimida and Triplonchida
5. Complete examination of nematodes from lab 6.
6. Videotape: Xiphinema index feeding on roots of seedlings.
7. Study photographs of characteristics and damage by migratory ectoparasites: Xiphinema, Longidorus and Trichodorus. Also, slides of the ultrastructure of these nematodes.
8. Review of use of Nemaplex for study of nematode characteristics and host ranges.
9. Homework Assignment:
NEMAPLEX Exercise
Main Menu:
Select...Biology and Taxonomy
Select..Phylogenetic Classification
Select...Order Dorylaimida
Review characteristics of this Order.
Select...Longidoridae
Review Longidorus, Paralongidorus, Xiphinema,
including some of the species.
Return to Nemaplex Main Menu
Select...Order Triplonchida
Review characteristics of this Order.
Select...Trichodoridae
Review Trichodorus, Paratrichodorus,
including some of the species.
Return to
Main Menu
Select...General Nematology
Select...Nematodes as Plant Parasites
Select...Nematodes as Virus Vectors
Return to
Main Menu
10. Nemabase Exercise
Review the host range of
these nematodes. What are the indicated non-hosts? Are there
resistant cultivars available?
From the Nemaplex main
Menu, select Management and Decision Support, then Nemabase 2012.
Alternatively, you can access the data from the descriptive page for each nematode.